HUMANIZED MODEL OF ISOLATED SUSPENSION CULTIVATION OF HEMATOPOIETIC PROGENITOR CELLS FOR THE INVESTIGATION OF IONIZING RADIATION INFLUENCE IN VIVO

Abstract

Objective: development of the humanized system for cells cultivation outside the human organism (human–mouse) and investigation of the influence of ionizing radiation in increasing doses on the colony-forming ability of hematopoietic progenitor cells. Materials and methods. Bone marrow samples of individuals without blood system diseases were cultivated in gel diffusion chambers with semi-solid agar in the abdominal cavity of CBA mice exposed to ionizing radiation action. Cell aggregates, which were obtained in the culture of diffusion chambers in vivo, were counted and colony-forming efficiency of bone marrow cells was determined. Results. We revealed the stimulation of colony forming under the action of ionizing radiation in increasing doses on the animals-recipients of the chambers, which indirectly indicates the synthesis of colony-stimulating factor in the mice organism and its permeation into the diffusion chambers with human bone marrow cells. The effect of cytostatics action on the mice organism was investigated, which in experimentally selected dose cause stimulation of colony forming in cell cultures, both 24 hours and 2 hours after administration. Conclusions. The ability of hematopoietic progenitor cells of bone marrow to form colonies and clusters was evaluated during the cultivation in semi-solid agar in gel diffusion chambers in vivo, as well as the association with the number of explanted cells in the appropriate range was established, which indicates the clonal nature of cell aggregates growth in culture. It was shown that the treatment of animals the day prior to experiment with administration of cytostatics is comparable to the action of ionizing radiation and can be used to study hematopoiesis in «human–mouse» system. Key words: hematopoietic progenitor cells, internal roentgen radiation, cytostatics, cell culture in gel diffusion chambers in «human–mouse» system.