Tumor Florescence Imaging Method Using 5-ALA in Ht-29 Cells

Abstract

Objective: 5-ALA, also known as 5-aminolevulinic acid, is a naturally occurring amino acid that plays a crucial role in the biosynthesis of heme, a vital component of hemoglobin and various enzymes in the body. Specifically, it is used in technique called photodynamic diagnosis (PDD) and photodynamic therapy (PDT) as a photosensitizer. When exposed to a specific wavelength of light, the accumulated 5-ALA causes fluorescence in these target areas, allowing for enhanced visualization and detection during surgical procedures, such as in neurosurgery or urology. The purpose of this study was to evaluate the conditions for optimal photodynamic diagnosis using 5-ALA in colon cancer. Methods: HT-29 cell line which was administered different 5-ALA (100, 200, 300, 500, 1000, 1500 μM) concentrations for 3 hours incubation time, were performed on cell viability, fluorescence intensity, apoptosis analysis. Results: While there was no difference in cell viability between the control group and low 5-ALA doses (100,200 and 300 μM), a significant difference was found at higher 5-ALA doses (1000 and 1500 μM) (p<,0001). 5-ALA doses increased in parallel with the fluorescence intensity, and the highest fluorescence intensity was at 1500 μM 5-ALA (p<,0001). The apoptosis/dead ratio was significantly showed to be highest at 1000 μM and 1500 μM 5-ALA which had the highest fluorescence intensity (p<0.05). Conclusion: the optimum dose of 5-ALA concentration was determined to be 500 μM in HT-29 cells. High concentrations of 5-ALA have been shown to cause apoptosis in HT29 cells.