Analysis of Frozen Bull Spermatozoa After Direct Washing with The Brackett Oliphant Medium

Abstract

Aim to study: The purpose of this study was to investigate the effects of the method of direct washing with the Brackett Oliphant (BO) medium which is used for purposes of in vitro fertilization on some spermatological parameters in Holstein and Brown Swiss bulls. Material and methods: The study used cryopreserved sperm obtained from the Holstein (n=5) and Brown Swiss (n=5) breeds. After this procedure, Computer Assisted Sperm Analysis (CASA) and oxidative status analyses were conducted both before and after direct washing method with BO. The data were analyzed using the statistical methods of paired-samples t-test and independent-samples t-test. Results: After direct washing with BO, the total motility in the Brown Swiss group decreased from 62.67 to 33.33 (P=0.011). After washing with BO, the total antioxidant level decreased from 1.45 to 0.11 in Group 1 (P = 0.000) and from 0.83 to 0.09 in Group 2 (P=0.000). Additionally, the total oxidant level increased from 5.54 to 5.70 in Group 1 (P = 0.024) and from 4.94 to 5.12 in Group 2 (P=0.019). Conclusion: According to the findings, the direct washing method with BO can negatively affect Brown Swiss spermatozoa motility. Additionally, after washing, the antioxidant level significantly decreases, and the oxidant levels increase due to oxidative stress.