Spectrophotometric Determination of Remdesivir Using the Ion Pair Complex Method Using through Alizarin Red S Dye

Abstract

The research included the development of a simple and practical spectroscopic method for the estimation of remdesivir (RDV) by the ion-pair method, which is based on the interaction of RDV with Alizarin Red S (ARS) at laboratory temperature. Using water as a medium for the reaction, a product Red in color was formed, with a linear relation ranging from 1 to 25 ?g/ml. It gave the highest absorption at wavelength 524 nm, and the complex completed its reaction after an hour, as the value of the sample was a relative standard deviation of no more than 1%, the recovery rate is 100.2429%, and high sensitivity to low concentrations, where she was the values of limit of detection, limit of quantitation were 0.0198, 0.0602 ?g/ml, respectively, detection limit of 0.0139 ?g/mL, and a quantitation limit of 0.0422 ?g/mL. The molar absorptivity is 51340.668 L/mol/cm, and Sandell?s sensitivity value is 0.0117 ?g.cm-2. The recovery values ranged from 99.3088% to 100.5184%, with a relative standard deviation not exceeding 0.7886% for RDV. The method was successfully applied for the estimation of pharmaceutical preparations using both direct and standard addition methods.. KEYWORDS :Alizarin red S, Ion pair, Remdesivir, Spectrophotometric estimation.