Isolation of lignocellulolytic bacteria from oil palm mesocarp waste

Author:

P VEERA LAKSHMI ,K PARANI

Abstract

In the present investigation, the bacterial strains were isolated from oil palm mesocarp waste dumped soil by serial dilution and pour plate method. The highest lignocellulolytic index was produced by bacterial isolate OPMW 6 (1.5 mm) followed by OPMW 5 (1.2 mm), OPMW 1 (0.83 mm), OPMW3 and OPMW 4 (0.71 mm). Changes in methylene blue color supplemented in nutrient broth from blue to light green or yellow were observed by three bacterial isolates (OPMW1, OPMW 3 and OPMW5), and rest of the bacterial isolatesrecorded less decolourization as the cultures were incubated between 24 and 48 hours respectively with highest percentage of dye decolourization produced by bacterial isolate i.e. OPMW5 (83.3%) and lowest rate ofdecolourization by bacterial isolate OPMW 6 (13.54%) as inoculated in nutrient broth supplemented with methylene blue dye. Utilization of gallic acid as supplemented in minimal salt media recorded positive reactionfor 4 bacterial isolates (OPMW 2, OPMW 5, OPMW 6 and OPMW 8) by the formation of dark colouration and addition ofa-naphthol in MSM inoculated broth culturesshowed formation of purple blue colour due to productionof laccase activity. Among the 4 best isolates having the highest indices, two isolates of bacteria (OPMW5 and OPMW6) had the best lignocellulolytic activity. Based on enzyme biosynthesis ability, the OPMW5 and OPMW6were selected as the best isolates as it can synthesie cellulase and ligninase enzymes. These enzymes are known to accelerate the degradation of oil palm mesocarp fibre substrate.

Publisher

Indian Society of Oilseeds Research

Reference14 articles.

1. Ariana C K P 2017. Isolation and characterization of lignocellulolytic microbes from oil palm empty fruit bunches (EFB). Journal of Environment and Sustainability, 1(1): 1-9.

2. Atlas R M and Bartha R 1986. Microbial Ecology: Fundamentals and Applications, Menlo Park:Benjamin-Cummings Pub. Co.

3. Bholay A D 2012. Bacterial lignin peroxidise: A tool for biobleaching and biodegradation of industrial effluents. Journal of Environment Resource Technology, 2(1): 58-64.

4. Chen Y, Chai L, Tang C, Yang Z, Zheng Y, Shi Y and Zhang H 2012. Kraft lignin biodegradation by Novosphingobium sp. B-7 and analysis of the degradation process. Bioresource Technology, 123: 682.

5. Gusma W 1999. Pengaruh pemberian mikroorganisme selulolitik dan kotoran ayam terhadap dekomposisi tandan kosong kelapa sawit, Universitas Sumatera Utara.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3