Abstract
Biological nitrogen fixation (BNF) is an important nitrogen source, providing a variety
of legumes and pasture plants. Rhizobia is soil bacteria that can form nitrogen-fixing
nodules on legumes. In this study, we have isolated 10 bacteria from root nodules of
sugar beans from the Gembos plain Derebucak district of Konya, Turkey. The
morphological and metabolic characteristics of the isolates were tested under
laboratory conditions. According to molecular identification, eight bacterial isolates
were identified as Agrobacterium tumefaciens, and two isolates (F4DC and F6DC) were
identified as Rhizobium gallicum. Field experiments were carried out to compare the
effect of one native rhizobia (Rhizobium gallicum F4DC), chemical fertilizer, and nonfertilizer control in SFWRRI Sarayköy Research and Application Station in Ankara. Our
results show that sugar bean (Phaseolus vulgaris L.) inoculation with Rhizobium
gallicum F4DC (MZ156852) induced a significant increase in the number of nodules,
grain yield, number of pods, and plant height compared to the control. These results
show that Rhizobium gallicum F4DC is a suitable choice for use in symbiotic association
with beans to work as a biofertilizer.
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