The structure of hemostatic aggregate and the assessment of platelet functional activity using flow cytometry-Reference-Cited by-同舟云学术

The structure of hemostatic aggregate and the assessment of platelet functional activity using flow cytometry

Published:2024-07-22 Issue:2 Volume:23 Page:192-197
ISSN:2414-9314
Container-title:Pediatric Hematology/Oncology and Immunopathology
language:
Short-container-title:Voprosy gematologii/onkologii i immunopatologii v pediatrii

Author:

Balandina A. N.¹^ORCID,Kuprash A. D.¹^ORCID,Nikitin N. S.²^ORCID,Kovalenko T. A.¹^ORCID,Ignatova A. A.¹^ORCID,Ataullakhanov F. I.³,Panteleev M. A.³^ORCID

Affiliation:

1. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation; Center for Theoretical Problems of Physical and Chemical Pharmacology, Russian Academy of Sciences

2. Center for Theoretical Problems of Physical and Chemical Pharmacology, Russian Academy of Sciences; N.I. Pirogov Russian National Research Medical University of Ministry of Healthcare of the Russian Federation

3. Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology of Ministry of Healthcare of the Russian Federation; Center for Theoretical Problems of Physical and Chemical Pharmacology, Russian Academy of Sciences; N.I. Pirogov Russian National Research Medical University of Ministry of Healthcare of the Russian Federation; M.V. Lomonosov Moscow State University

Abstract

A thrombus is a heterogeneous structure consisting of platelets in different functional states. Flow cytometry is one of the most promising tools for the diagnosis of platelet state. However, its optimization and standardization are the subjects of heated debate. How to properly activate and label platelets in order to assess their functional status? In this work, we would like to briefly highlight this issue and propose the hypothesis that several levels/types of platelet activation correspond to various positions in the thrombus and various physiological meanings. One should use this entire necessary and sufficient set of activation levels in order to draw a conclusion about how the patient’s platelets “feel”.

Publisher

Fund Doctors, Innovations, Science for Children

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