Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge

Author:

Munkhbayar TsolmonORCID,Gachmaa BatzayaORCID,Jukov AzzayaORCID,Yungeree OyunbilegORCID

Abstract

In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether the content of biologically active compounds depends on the stage of development of the plant during in vitro culture. The content of total saponins and polysaccharides in different cultures of A. mongholicus grown in vitro was higher (990 and 505 μg/ml) in ethanol extracted 14-day-old young shoot samples than in 28-day-old propagated shoot samples and rooted shoots.

Publisher

Mongolian Journals Online

Subject

Automotive Engineering

Reference38 articles.

1. Basalma, D., Uranbey, S., Gürlek, D. and Özcan, S. (2008). TDZ-induced Plant Regeneration in Astragalus cicer L. Afr. J. Biotechnol., 7(8): pp. 955-959.

2. Edreva. A., Velikova. V., Tsonev. T. (2008). Stress-protective role of secondary metabolites: diversity of functions and mechanisms. Gen Appl Plant Physiol. 34(1–2):67–78.

3. Edson JL, Wenny DL, Leege-Brusven A, Everett RL & Henderson DM (1994). Conserving threatened rare plants: some nursery strategies. National Proceedings, Forest and Conservation Nursery Associations. Fort Collins, CO: USDA For. Serv., Rocky Mtn. Forest and Range Exp. Sta., Gen. Tech. Rep. RMGTR-257:150-157.

4. Erisen, S., Yorgancilar, M., Atalay, E. and Babaoglu, M. (2009). Prolific Shoot Regeneration of Astragalus cariensis Boiss. Plant. Cell. Tiss. Org., 100(2):229–233. https://doi.org/10.1007/s11240-009-9638-3

5. Erisen, S., Yorgancilar, M., Atalay, E., Babaoglu, M. and Duran, A. (2010). Callus Induction and Plant Regeneration of the Endemic Astragalus nezaketae in Turkey. Electron. J. Biotechnol., 13(6):13-14. https://doi.org/10.2225/vol13-issue6-fulltext-3

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