The correlation analysis of miRNAs and target genes in metastasis of cervical squamous cell carcinoma

Abstract

Aim: This study was intended to identify the metastasis-related miRNAs and target genes in cervical squamous cell carcinoma. Materials & methods: The mRNA and miRNA next-generation sequencing data were downloaded. Differential expression analysis was carried out, followed by target gene prediction of differentially expressed miRNAs. The biological function of differentially expressed genes was performed. Validation was carried out by survival analysis and qRT-PCR. Results: N4BP3 were associated with the survival time of patients. Hsa-mir-451 and hsa-mir-486 were related to tumor differentiation stage. Validated expression of hsa-mir-24–2, hsa-mir-582, NOTCH1, PIP4K2B, DIP2B and IGFBP5 was consistent with the bioinformatics analysis. Conclusion: Alterations of miRNAs and target genes may be useful in understanding the metastasis mechanisms of cervical squamous cell carcinoma.