Imaging and organelle distribution of fluorescent InGaP/ZnS nanoparticles in glial cells-Reference-Cited by-同舟云学术

Imaging and organelle distribution of fluorescent InGaP/ZnS nanoparticles in glial cells

Published:2009-10 Issue:7 Volume:4 Page:747-761
ISSN:1743-5889
Container-title:Nanomedicine
language:en
Short-container-title:Nanomedicine

Author:

Behrendt Maik¹²,Sandros Marinella G³,McKinney R Anne¹,McDonald Ken⁴,Przybytkowski Ewa¹,Tabrizian Maryam³,Maysinger Dusica¹

Affiliation:

1. Department of Pharmacology & Therapeutics, McGill University, 3655 Promenade Sir-William-Osler, Montreal, Quebec, H3G 1Y6, Canada.

2. Department of Biomedicine, Institute of Anatomy, University of Basel, CH-4056 Basel, Switzerland

3. Department of Biomedical Engineering, McGill University, 3775 University Street, Montreal, Quebec, H3A 2B4, Canada

4. McGill Flow Cytometry Facility, McGill University, 3649 Promenade Sir-William-Osler, Montreal, Quebec, H3G 0B1, Canada

Abstract

Aim: To assess the effects of oleic acid treatment on subcellular distribution of indium gallium phosphide–zinc sulfide (InGaP/ZnS) nanoparticles in microglia and astrocytes. Materials & methods: The extent of colocalization between the nanoparticles and organelles was assessed by confocal microscopy, spectrofluorometry and cell sorting. Results: Cell treatment with a common fatty acid (oleic acid) within the range of physiological concentrations markedly enhanced the InGaP/ZnS uptake by microglia and afforded their colocalization within lipid droplets/lysosomes but not with mitochondria. Conclusion: These results suggest that the availability of mono-unsaturated fatty acids, such as oleic acid, in different cells could significantly alter nanoparticle uptake and localization, which can in turn affect the functions of cells and tissues coexposed to nanoparticles.

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/nnm.09.64

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