Inorganic nanoparticles as potential regulators of immune response in dendritic cells

Author:

Fogli Silvia1,Montis Costanza1,Paccosi Sara2,Silvano Angela3,Michelucci Elena4,Berti Debora1,Bosi Alberto3,Parenti Astrid2,Romagnoli Paolo3

Affiliation:

1. Department of Chemistry ‘Ugo Schiff,’ University of Florence, and CSGI, via della Lastruccia 3, 50019 Sesto Fiorentino, FI, Italy

2. Department of Health Sciences, Clinical Pharmacology & Oncology Section, University of Florence, Viale Pieraccini 6, 50139, FI, Italy

3. Department of Experimental & Clinical Medicine, University of Florence, via Ugo Schiff 6, 50019 Sesto Fiorentino, FI, Italy

4. Mass Spectrometry Center (CISM), University of Florence, via Ugo Schiff 6, 50019 Sesto Fiorentino, FI, Italy

Abstract

Aim: The spontaneous adsorption of proteins on nanoparticles (NPs) in biological media is exploited to prepare complexes of NPs and proteins from cancer cells’ lysates for application in cancer immunotherapy. Materials & methods: Gold (Au) and silica NPs were synthesized, incubated with cancer cells’ lysates and characterized. Dendritic cells (DCs) were challenged with protein-coated NPs, their maturation, viability and morphology were evaluated and lymphocytes T proliferation was determined. Results: Silica and Au NPs bound different pools of biomolecules from lysates, and are therefore promising selective carriers for antigens. When incubated with immature DCs, NPs were efficiently endocytosed without cytotoxicity. Finally, protein-coated AuNPs promoted DC maturation and DC-mediated lymphocyte proliferation, at variance with lysate alone and protein-coated silica NPs, that did not promote DCs maturation. Conclusion: These results demonstrate that the spontaneous formation of protein coronas on NPs represents a possible approach to fast, easy, cost-effective DCs stimulation.

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

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