The CRISPR-cas system promotes antimicrobial resistance in Campylobacter jejuni-Reference-Cited by-同舟云学术

The CRISPR-cas system promotes antimicrobial resistance in Campylobacter jejuni

Published:2018-12 Issue:16 Volume:13 Page:1757-1774
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Shabbir Muhammad AB¹,Wu Qin²,Shabbir Muhammad Z³,Sajid Abdul¹⁴,Ahmed Saeed¹,Sattar Adeel⁵,Tang Yanping¹,Li Jun¹,Maan Muhammad K¹,Hao Haihong¹²,Yuan Zonghui¹²

Affiliation:

1. China MOA Laboratory for Risk Assessment of Quality & Safety of Livestock & Poultry Products, Huazhong Agricultural University, Wuhan 430070, PR China

2. National Reference Laboratory of Veterinary Drug Residues & MOA Key Laboratory for the Detection of Veterinary Drug Residues in Foods, Huazhong Agricultural University, Wuhan 430070, PR China

3. Quality Operation Laboratory at University of Veterinary & Animal Sciences, Lahore, 54600, Pakistan

4. College of Veterinary Sciences & Animal Husbandry, Abdul Wali Khan University, Mardan, 23200, Pakistan

5. Department of Pharmacology & Toxicology, University of Veterinary & Animal Sciences, Lahore, 54600, Pakistan

Abstract

Aim: The purpose of current study is to find out relationship between cas9 gene and antimicrobial resistance in Campylobacter jejuni NCTC11168. Materials & methods: The involvement of the cas9 gene in antimicrobial resistance of C. jejuni was determined by assessment of minimum inhibitory concentration, clustered regularly interspaced short palindromic repeats (CRISPR)-cas gene expression in standard strains, in vitro resistance development and transcriptome analysis of a cas9 deletion mutant and wild strains. Results: Increased expression of CRISPR-related genes was observed in standard strains. We also observed that Δcas9 mutant strain is more sensitive to antibiotics than its wild strain. Transcriptome analysis revealed that cas9 gene regulate several genes to promote antimicrobial resistance in C. jejuni. Conclusion: CRISPR-cas system plays role in the enhancement of antimicrobial resistance in C. jejuni.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2018-0234

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