The effect of mesenchymal stem cell-secreted factors on airway epithelial repair

Abstract

Aim: This study was aimed to investigate the effect of mesenchymal stem cell (MSC)-secreted factors on airway repair. Materials & methods: An indirect in vitro coculture model of injured airway epithelium explant with MSCs was developed. LC–MS/MS analysis was performed to determine factors secreted by MSCs and their involvement in epithelium repair was evaluated by histopathological assessment. Results: The identification of 54 of MSC proteins of which 44 of them were secretory/extracellular proteins. 43 of the secreted proteins were found to be involved in accelerating airway epithelium repair by stimulating the migratory, proliferative and differentiation abilities of the endogenous repair mechanisms. MSC-secreted proteins also initiated epithelial–mesenchymal transition process during early repair. Conclusion: MSC-secreted factors accelerated airway epithelial repair by stimulating the endogenous reparative and regenerative ability of lung cells.