shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats-Reference-Cited by-同舟云学术

shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats

Published:2015-05 Issue:4 Volume:10 Page:411-429
ISSN:1746-0751
Container-title:Regenerative Medicine
language:en
Short-container-title:Regenerative Medicine

Author:

Zhou Hengxing¹,Li Xueying²,Wu Qiang³,Li Fuyuan¹,Fu Zheng²,Liu Chang⁴,Liang Zhipin⁴,Chu Tianci¹,Wang Tianyi¹,Lu Lu¹,Ning Guangzhi¹,Kong Xiaohong⁴,Feng Shiqing¹

Affiliation:

1. Department of Orthopaedics, Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, PR China

2. Key Laboratory of Immuno Microenvironment & Disease of the Educational Ministry of China, Department of Immunology, Tianjin Medical University, No. 22 Qixiangtai Road, Heping District, Tianjin 300070, PR China

3. Department of Orthopaedics, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314 Anshanxi Road, Nankai District, Tianjin 300193, PR China

4. School of Medicine, Nankai University, No. 94 Weijin Road, Nankai District, Tianjin 300071, PR China

Abstract

Aim: To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi. Materials & methods: Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed. Results: Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation. Conclusions: PTEN silencing may be promising for spinal cord injury repair.

Publisher

Future Medicine Ltd

Subject

Embryology,Biomedical Engineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/rme.14.88

Reference56 articles.

1. Global Incidence and Prevalence of Traumatic Spinal Cord Injury

2. Role of extracellular factors in axon regeneration in the CNS: implications for therapy

3. Neuronal intrinsic barriers for axon regeneration in the adult CNS

4. Enhancing intrinsic growth capacity promotes adult CNS regeneration

5. Glial inhibition of CNS axon regeneration

Cited by 11 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. PTEN knockout using retrogradely transported AAVs transiently restores locomotor abilities in both acute and chronic spinal cord injury;Experimental Neurology;2023-10

2. PTEN knockout using retrogradely transported AAVs restores locomotor abilities in both acute and chronic spinal cord injury;2023-04-17

3. The potential of gene therapies for spinal cord injury repair: a systematic review and meta-analysis of pre-clinical studies;Neural Regeneration Research;2023

4. Gypenoside XVII protects against spinal cord injury in mice by regulating the microRNA‑21‑mediated PTEN/AKT/mTOR pathway;International Journal of Molecular Medicine;2021-06-04

5. PTEN modulates neurites outgrowth and neuron apoptosis involving the PI3K/Akt/mTOR signaling pathway;Molecular Medicine Reports;2019-09-10