1α,25-dihydroxyvitamin D3-eluting nanofibrous dressings induce endogenous antimicrobial peptide expression

Author:

Jiang Jiang1,Zhang Yang23,Indra Arup K2456,Ganguli-Indra Gitali46,Le Mai N4,Wang Hongjun1,Hollins Ronald R7,Reilly Debra A7,Carlson Mark A89,Gallo Richard L10,Gombart Adrian F23,Xie Jingwei1

Affiliation:

1. Department of Surgery, Transplant & Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE 68198, USA

2. Department of Biochemistry & Biophysics, Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA

3. Nutrition Graduate Program, School of Biological & Population Health Sciences, College of Public Health & Human Sciences, Oregon State University, Corvallis, OR 97331, USA

4. Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, OR 97331, USA

5. Department of Dermatology, Oregon Health & Science University (OHSU), Portland, OR 97239, USA

6. Knight Cancer Institute, OHSU, Portland, OR 97239, USA

7. Department of Surgery – Plastic & Reconstructive Surgery, University of Nebraska Medical Center, Omaha, NE 68198, USA

8. Department of Surgery – General Surgery & Genetics, Cell Biology & Anatomy, University of Nebraska Medical Center, Omaha, NE 68198, USA

9. Department of Surgery, VA Nebraska – Western Iowa Health Care System, Omaha, NE 68105, USA

10. Department of Dermatology, University of California, San Diego, CA 92093, USA

Abstract

Aim: The aim of this study was to develop a nanofiber-based dressing capable of local sustained delivery of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3) and augmenting human CAMP induction. Materials & methods: Nanofibrous wound dressings containing 1,25(OH)2D3 were successfully prepared by electrospinning, which were examined in vitro, in vivo and ex vivo. Results: 1,25(OH)2D3 was successfully loaded into nanofibers with encapsulation efficiency larger than 90%. 1,25(OH)2D3 showed a sustained release from nanofibers over 4 weeks. Treatment of U937 and HaCaT cells with 1,25(OH)2D3-loaded poly(ϵ-caprolactone) nanofibers significantly induced hCAP18/LL37 expression in monocytes and keratinocytes, skin wounds of humanized transgenic mice and artificial wounds of human skin explants. Conclusion: 1,25(OH)2D3 containing nanofibrous dressings could enhance innate immunity by inducing antimicrobial peptide production.

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

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