The patch assay reconstitutes mature hair follicles by culture-expanded human cells

Author:

Zhang Qun12,Zu Tingjian2,Zhou Qian2,Wen Jie2,Leng Xue2,Wu Xunwei12

Affiliation:

1. Suzhou Institute of Shandong University, Building H of NUSP, 388 Ruoshui Road, Suzhou Industrial Park, Suzhou, Jiangsu, China

2. School of Stomatology, Shandong University, Jinan, Shandong China

Abstract

Aim: We tested whether the a simple injection known as the patch assay could reconstitute mature hair follicles by culture-expanded human cells and explored whether the assay could reflect the trichogenicity of cultured cells. Materials & methods: Dissociated culture-expanded fetal or adult scalp dermal cells combined with foreskin keratinocytes were subcutaneously injected into the back skin of immunosuppressive mice to form the patch skin. The patches were collected and characterized and were analyzed for hair formation efficiency. Results: Using culture-expanded human fetal cells, the patch assay can efficiently reconstitute mature hair follicles and the efficiency of hair formation in the patch assay correlates with cell trichogenicity. Conclusion: The patch assay has the potential for testing the trichogenicity of human cells.

Publisher

Future Medicine Ltd

Subject

Embryology,Biomedical Engineering

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