Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles-Reference-Cited by-同舟云学术

Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles

Published:2023-03 Issue:6 Volume:18 Page:541-554
ISSN:1743-5889
Container-title:Nanomedicine
language:en
Short-container-title:Nanomedicine

Author:

Picheth Guilherme F¹²^ORCID,Cardoso Gabriela C¹,Collini Michelle B¹,Filizzola João OC¹,Colauto Leonardo B¹,Nunes Giovana G³,Pirich Cleverton L⁴,da Silva Thiago Alessandre⁴,Pontes Flávia LD²,Zattoni Ingrid F⁵,Cavalieri Edneia ASR¹,Klassen Giseli¹

Affiliation:

1. Department of Basic Pathology, Federal University of Paraná, Curitiba, Paraná, Brazil

2. Department of Biochemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

3. Department of Chemistry, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

4. Department of Bioprocess Engineering & Biotechnology, Federal University of Paraná, Curitiba, Paraná, 8150-000, Brazil

5. School of Medicine, Pontifical Catholic University of Paraná. Curitiba, Paraná, 80215-901, Brazil

Abstract

Aims: Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the demethylating agent decitabine. Materials & methods: The polymers were synthesized by ring-opening polymerization of D,L-lactide and formulated into nanoparticles via emulsion-evaporation method. The nanoparticles were characterized by physicochemical analysis as well as in vitro using breast cancer cell lineages. Results & conclusion: The targeted nanoparticles exhibited a hydrodynamic diameter of 75 ± 12 nm, zeta potential -6.3 ± 0.2 mV and spherical morphology, and displayed greater in vitro accumulation into MDA-MB-231 (triple-negative breast cancer cell-line) compared with MCF7 and HB4A cell lineages as verified by fluorescence confocal microscopy and significant demethylating effects via ADAM33 screening by PCR.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/nnm-2023-0006

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