Comparison of smoking-related DNA methylation between newborns from prenatal exposure and adults from personal smoking

Author:

Sikdar Sinjini1,Joehanes Roby23ORCID,Joubert Bonnie R4,Xu Cheng-Jian56ORCID,Vives-Usano Marta78910,Rezwan Faisal I11,Felix Janine F121314,Ward James M1,Guan Weihua15,Richmond Rebecca C16,Brody Jennifer A17,Küpers Leanne K161819,Baïz Nour20,Håberg Siri E21,Smith Jennifer A22,Reese Sarah E1,Aslibekyan Stella23,Hoyo Cathrine24,Dhingra Radhika2526,Markunas Christina A27,Xu Tao28,Reynolds Lindsay M29,Just Allan C30,Mandaviya Pooja R3132,Ghantous Akram33,Bennett Brian D1,Wang Tianyuan1ORCID,Consortium The BIOS,Bakulski Kelly M22,Melen Erik34,Zhao Shanshan1,Jin Jianping35,Herceg Zdenko33,Meurs Joyce van31,Taylor Jack A1,Baccarelli Andrea A36,Murphy Susan K37,Liu Yongmei29,Munthe-Kaas Monica Cheng3839,Deary Ian J4041,Nystad Wenche42,Waldenberger Melanie2843,Annesi-Maesano Isabella20,Conneely Karen44,Jaddoe Vincent WV121314,Arnett Donna23,Snieder Harold18,Kardia Sharon LR22,Relton Caroline L16,Ong Ken K4546,Ewart Susan47,Moreno-Macias Hortensia48,Romieu Isabelle495051,Sotoodehnia Nona17,Fornage Myriam52,Motsinger-Reif Alison1,Koppelman Gerard H56,Bustamante Mariona78910,Levy Daniel353,London Stephanie J1

Affiliation:

1. Department of Health & Human Services, Division of Intramural Research, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

2. Hebrew SeniorLife, Harvard Medical School, Boston, MA 02115, USA

3. Framingham Heart Study, Framingham, MA 01702, USA

4. Department of Health & Human Services, Division of Extramural Research & Training, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA

5. Department of Pediatric Pulmonology & Pediatric Allergology, Beatrix Children’s Hospital, University of Groningen, University Medical Center Groningen, PO Box 30001, Groningen, The Netherlands

6. GRIAC Research Institute Groningen, University of Groningen, University Medical Center Groningen, PO Box 30001, Groningen, The Netherlands

7. ISGlobal, Barcelona Institute for Global Health, Barcelona, Spain

8. Universitat Pompeu Fabra (UPF), Barcelona, Spain

9. CIBER Epidemiología y Salud Pública (CIBERESP), Barcelona, Spain

10. Center for Genomic Regulation (CRG), Barcelona Institute of Science & Technology, Barcelona, Spain

11. Human Development & Health, Faculty of Medicine, University of Southampton, Southampton, UK

12. The Generation R Study Group, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands

13. Department of Pediatrics, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands

14. Department of Epidemiology, Erasmus Medical Center, University Medical Center Rotterdam, Rotterdam, The Netherlands

15. Division of Biostatistics, School of Public Health, University of Minnesota, Minneapolis, MN 55455, USA

16. MRC Integrative Epidemiology Unit, School of Social & Community Medicine, University of Bristol, Bristol, UK

17. Department of Medicine, Epidemiology, & Health Services, Cardiovascular Health Research Unit, University of Washington, Seattle, WA 98101, USA

18. Department of Epidemiology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

19. Division of Human Nutrition & Health, Wageningen University, Wageningen, The Netherlands

20. Epidemiology of Allergic & Respiratory Diseases Department (EPAR), Sorbonne Universités, INSERM, Pierre Louis Institute of Epidemiology & Public Health (IPLESP UMRS 1136), Saint-Antoine Medical School, Paris, France

21. Centre for Fertility & Health, Norwegian Institute of Public Health, Oslo, Norway

22. Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA

23. College of Public Health, University of Kentucky, Lexington, KY 40536, USA

24. Department of Biological Sciences & Center for Human Health & the Environment, North Carolina State University, Raleigh, NC 27695, USA

25. Department of Environmental Sciences & Engineering, University of North Carolina Gillings School of Global Public Health, Chapel Hill, NC 27599, USA

26. Institute for Environmental Health Solutions, University of North Carolina, Chapel Hill, NC 27599, USA

27. Behavioral Health Research Division, RTI International, Research Triangle Park, NC 27709, USA

28. Research Unit of Molecular Epidemiology, Helmhotz Zentrum Muenchen, Munich, Germany

29. Department of Epidemiology & Prevention, Public Health Sciences, Wake Forest School of Medicine, Winston-Salem, NC 27101, USA

30. Department of Environmental Medicine & Public Health, Icahn School of Medicine at Mount Sinai, New York City, NY 10029, USA

31. Department of Internal Medicine, Erasmus University Medical Center, Rotterdam, The Netherlands

32. Department of Clinical Chemistry, Erasmus University Medical Center, Rotterdam, The Netherlands

33. Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France

34. Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden

35. Westat, Durham, NC 27703, USA

36. Department of Environmental Health Sciences, Columbia University Mailman School of Public Health, New York City, NY 10032, USA

37. Departments of Obstetrics & Gynecology & Pathology, Duke University School of Medicine, Durham, NC 27708, USA

38. Department of Pediatrics, Oslo University Hospital, Oslo, Norway

39. National Institute of Public Health, Oslo, Norway

40. Centre for Cognitive Ageing & Cognitive Epidemiology, Edinburgh, UK

41. Department of Psychology, University of Edinburgh, Edinburgh, UK

42. Division of Mental & Physical Health, Norwegian Institute of Public Health, Oslo, Norway

43. Institute of Epidemiology, Helmholtz Zentrum München, German Research Center for Environmental Health, Neuherberg, Germany

44. Department of Human Genetics, Emory University School of Medicine, Atlanta, GA 30322, USA

45. MRC Epidemiology Unit, Institute of Metabolic Science, University of Cambridge, Cambridge, UK

46. Department of Paediatrics, University of Cambridge, Cambridge, UK

47. Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA

48. Autonomous Metropolitan University Iztapalapa, Mexico City, Mexico

49. Nutrition & Metabolism Section, International Agency for Research on Cancer, Lyon, France

50. Center for Research on Population Health, National Institute of Public Health, Mexico

51. Hubert Department of Global Health, Emory University, Atlanta, GA 30329, USA

52. Institute of Molecular Medicine & Human Genetics Center, University of Texas Health Science Center at Houston, Houston, TX 77225, USA

53. Population Sciences Branch, National Heart, Lung, & Blood Institute, National Institutes of Health, Bethesda, MD 01702, USA

Abstract

Aim: Cigarette smoking influences DNA methylation genome wide, in newborns from pregnancy exposure and in adults from personal smoking. Whether a unique methylation signature exists for in utero exposure in newborns is unknown. Materials & methods: We separately meta-analyzed newborn blood DNA methylation (assessed using Illumina450k Beadchip), in relation to sustained maternal smoking during pregnancy (9 cohorts, 5648 newborns, 897 exposed) and adult blood methylation and personal smoking (16 cohorts, 15907 participants, 2433 current smokers). Results & conclusion: Comparing meta-analyses, we identified numerous signatures specific to newborns along with many shared between newborns and adults. Unique smoking-associated genes in newborns were enriched in xenobiotic metabolism pathways. Our findings may provide insights into specific health impacts of prenatal exposure on offspring.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

Reference43 articles.

1. National Center for Chronic Disease Prevention and Health Promotion, Office on Smoking and Health, Centers for Disease Control and Prevention. 2014 Surgeon General's Report: The Health Consequences of Smoking – 50 Years of Progress (2014). https://www.cdc.gov/tobacco/data_statistics/sgr/50th-anniversary/index.htm#report

2. Epigenetic Signatures of Cigarette Smoking

3. DNA Methylation in Newborns and Maternal Smoking in Pregnancy: Genome-wide Consortium Meta-analysis

4. Cigarette smoking and reproductive and developmental toxicity

5. Maternal Smoking and DNA Methylation in Newborns: In Utero Effect or Epigenetic Inheritance?

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