A new model of the mechanism underlying lead poisoning: SNPs in miRNA target region influence the δ-aminolevulinic acid dehydratase expression level

Abstract

Aim: To determine if SNPs located within the 3′-UTR of δ-aminolevulinic acid dehydratase (ALAD) can alter the risk of lead poisoning and the ALAD gene expression. Materials & methods: A case–control study was carried out to find the SNPs in miRNA target region. Luciferase reporter gene assay, qRT-PCR and Western blot was used to determine the relationship between miRNA and SNPs. Results: We found a significant association between rs818708 and the risk of lead poisoning. miR-545–5p was influenced by rs818708 variant and might result in a significant change in ALAD expression. Conclusion: rs818708 T > C can weaken the binding capability between miR-545–5p and 3′-UTR of ALAD and thus may alter the risk of lead poisoning.