Screening circular RNA related to chemotherapeutic resistance in osteosarcoma by RNA sequencing

Abstract

Aim: To identify circular RNAs (circRNAs) related to osteosarcoma (OS) chemoresistance. Materials & methods: CircRNA expression profile was performed in three paired human chemoresistant and chemosensitive OS cell lines by next-generation sequencing. Quantitative real-time-PCR (qRT-PCR) was used to confirm next-generation sequencing data. Bioinformatics analysis was conducted to predict their functions. Results: Eighty circRNAs were dysregulated in the chemoresistant OS cells compared with the control, after validated by qRT-PCR. Bioinformatics analysis showed that some pathways related to drug metabolism were significantly enriched. Additionally, hsa_circ_0004674 was distinctly increased in OS chemoresistant cells and tissues, related to poor prognosis. CircRNA-miRNA-mRNA pathways related to hsa_circ_0004674 were constructed by TargetScan and miRanda. Conclusion: CircRNAs may play a role in OS chemoresistance and hsa_circ_0004674 might be a candidate target.