Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use-Reference-Cited by-同舟云学术

Human-derived feeder fibroblasts for the culture of epithelial cells for clinical use

Published:2016-09 Issue:6 Volume:11 Page:529-543
ISSN:1746-0751
Container-title:Regenerative Medicine
language:en
Short-container-title:Regenerative Medicine

Author:

O'Callaghan Anna R¹,Morgan Louise¹,Daniels Julie T¹,Lewis Mark P²

Affiliation:

1. Department of Ocular Biology & Therapeutics, UCL Institute of Ophthalmology, University College London, London EC1V 9EL, UK

2. National Centre for Sport & Exercise Medicine (NCSEM), EPSRC-MRC Centre for Doctoral Training in Regenerative Medicine, School of Sport, Exercise & Health Sciences, Loughborough University, Leicestershire, LE11 3TU, UK

Abstract

Aim: To investigate human oral mucosal fibroblasts (HOMF) and human limbal fibroblasts (HLF) as alternatives to murine 3T3 feeder fibroblasts currently used to support epithelial cell expansion for the treatment of limbal epithelial stem cell deficiency. Methods: HLF and HOMF were compared with 3T3s for their ability to support the culture of human limbal epithelial cells and human oral mucosal epithelial cells. Results: HOMF, but not HLF, were equivalent to 3T3s in terms of the number of epithelial population doublings achieved. Human limbal epithelial cells co-cultured with HOMF or 3T3s had similar expression of corneal and putative stem cell markers. Conclusion: HOMF are a suitable and safer feeder fibroblast alternative to 3T3s for the production of epithelial cells for clinical use.

Publisher

Future Medicine Ltd

Subject

Embryology,Biomedical Engineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/rme-2016-0039

Reference32 articles.

1. Critical Appraisal of Ex Vivo Expansion of Human Limbal Epithelial Stem Cells

2. Culture of Oral Mucosal Epithelial Cells for the Purpose of Treating Limbal Stem Cell Deficiency

3. Localisation of Epithelial Cells Capable of Holoclone Formation In Vitro and Direct Interaction with Stromal Cells in the Native Human Limbal Crypt

4. A Novel NIH/3T3 Duplex Feeder System to Engineer Corneal Epithelial Sheets with Enhanced Cytokeratin 15-Positive Progenitor Populations

5. Modulation of Keratin and Connexin Expression in Limbal Epithelium Expanded on Denuded Amniotic Membrane with and without a 3T3 Fibroblast Feeder Layer

Cited by 14 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Adipose Mesenchymal Stem Cell Derived Exosomes Promote Keratinocytes and Fibroblasts Embedded in Collagen/Platelet‐Rich Plasma Scaffold and Accelerate Wound Healing;Advanced Materials;2023-08-09

2. Fibroblast activating protein promotes the proliferation, migration, and activation of fibroblasts in oral submucous fibrosis;Oral Diseases;2023-06-25

3. The use of human‐derived feeder layers for the cultivation of transplantable human epidermal cell sheet to repair second degree burn wounds;Skin Research and Technology;2023-02

4. The progress in techniques for culturing human limbal epithelial stem cells;Human Cell;2022-10-01

5. Human Oral Mucosal Fibroblasts from Limbal Stem Cell Deficient Patients as an Autologous Feeder Layer for Epithelial Cell Culture;Current Eye Research;2022-05-16