Sequence analysis of Epstein–Barr virus (EBV) BNLF2a gene in malignant hematopathy of Northern China

Abstract

BNLF2a is an early Epstein–Barr virus gene whose protein plays an immune escaping role by inhibiting the transporter associated with antigen processing. To explore the association between BNLF2a gene variations and EBV tumorigenesis, the BNLF2a gene of 259 EBV-positive samples (171 lymphohematopoietic disease samples and 88 throat washings from healthy donors) from northern China were sequenced. On the basis of phylogenetic tree and mutation characteristics of BNLF2a, all specimens were divided into two major genotypes: BNLF2a-A and BNLF2a-B. BNLF2a-A type, similar to the prototype B95-8, was the major subtype in all subpopulations. Healthy donors carried less BNLF2a-A and more BNLF2a-B than donors with lymphohematopoietic disease. The conservation of the BNLF2a gene may be crucial to its function.