Immuno- and bio-informatic analysis of hexon protein in human adenovirus D8 isolated from patients with keratoconjunctivitis

Abstract

Aim: In silico analysis of the hexon protein of human adenovirus serotype D-8 isolated from a patients with keratoconjunctivitis in Iran. Materials & methods: The hexon gene of HAdV-D8 was amplified by PCR. HAdV-D8 recovered from EKC outbreak was isolated by growing in A549 cells. Results: The hexon gene isolated from a patient with EKC comprised 2829 nt and 942 aa. The analyses of selected B-cell epitopes prediction (KTFQPEPQIGENNWQD) and T-cell epitopes prediction (TENFDIDLAFFDIPQ), showed high score immunogenicity, which may prove this to be a promising candidate for epitope vaccine development. Conclusion: In silico analysis of selected B-cell epitopes prediction (KTFQPEPQIGENNWQD) and T-cell epitopes prediction (TENFDIDLAFFDIPQ) are immunogenic and provoke B- and T-cell responses.