Genotyping and sero-virological characterization of hepatitis B virus-infected blood donors in Central Eritrea-Reference-Cited by-同舟云学术

Genotyping and sero-virological characterization of hepatitis B virus-infected blood donors in Central Eritrea

Published:2022-11 Issue:11 Volume:17 Page:807-817
ISSN:1746-0794
Container-title:Future Virology
language:en
Short-container-title:Future Virology

Author:

Hamida Mohammed Elfatih¹^ORCID,Raja Saud Mohammed²^ORCID,Petros Yodahi³,Wahab Munir³,Elkhidir Isam Mohammed⁴,Achila Oliver Okoth⁵^ORCID,Tekle Freweini⁶,Berhane Isayas Yohannes⁷

Affiliation:

1. Department of Microbiology, Orotta College of Medicine & Health Sciences, Asmara, Eritrea

2. Department of Internal Medicine, Orotta College of Medicine & Health Sciences, Asmara, Eritrea

3. National Animal & Plant Health Laboratory, Unit of Molecular Biology, Asmara, Eritrea

4. Department of Microbiology, University of Khartoum, Faculty of Medicine, Khartoum, Sudan

5. Department of Clinical Laboratory Sciences, Asmara College of Health Science (ACHS), Asmara, Eritrea

6. Ministry of Health, National Health Laboratory, Asmara, Eritrea

7. Data Management Unit, National Blood Transfusion Service (NBTS), Asmara, Eritrea

Abstract

Aim: To determine the serological markers and genotype profiles of hepatitis B virus (HBV) isolates in Central Eritrea. Materials & methods: A total of 191 hepatitis B surface antigen (HBsAg)-positive sera were randomly selected for the study. ELISA was used to perform HBV seromarker screening, genotypes were determined using multiplex-nested PCR. Results: Of 191, 77.5% (148/191) were positive for HBcAb (total), among which 99.3% (147/148) and 0.7% (1/148) were positive for HBsAg and hepatitis B surface antibody, respectively. Of the 147 positive HBcAb/HBsAg, 16 (10.9%) and 131 (77.9%) were positive for HBeAg and HBeAb, respectively. A total of 73 HBV isolates were successfully genotyped: 39 (53.4%) D; 10 (13.7%) E; 6 (8.2%) A; 6 (8.2%) C/D; 4 (5.5%) C; 3 (4.1%) C/D/E; 2 (2.7%) A/D; 2 (2.7%) D/E; and 1 (1.4%) B/D. Conclusion: HBV genotype D is the predominant genotype among blood donors in Eritrea.

Publisher

Future Medicine Ltd

Subject

Virology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fvl-2021-0069

Reference55 articles.

1. Estimations of worldwide prevalence of chronic hepatitis B virus infection: a systematic review of data published between 1965 and 2013

2. Epidemiology of hepatitis B virus in Africa, its genotypes and clinical associations of genotypes

3. Organization WH. Guidelines for the prevention, care, and treatment of persons with chronic hepatitis B infection. (2015). https://www.who.int/publications/i/item/9789241549059

4. History and Global Burden of Viral Hepatitis

5. Organization WH. WHO guidelines on hepatitis B and C testing. (2017). https://apps.who.int/iris/bitstream/handle/10665/254621/9789241549981-eng.pdf