The clinicopathological features and prognosis of tumor MSI in East Asian colorectal cancer patients using NCI panel

Abstract

Aim: To compare test results obtained from a PCR assay for the National Cancer Institute (NCI) five loci criteria for detecting microsatellite instability (MSI) with those obtained from immunohistochemistry of mismatch repair and a five-mononucleotide site amplification system in East Asian patients with colorectal cancer. Patients & methods: A total of 245 East Asian patients with colorectal cancer were studied retrospectively at our institution. Results: The consistency of the NCI panel PCR method compared with detection of mismatch repair protein expression by immunohistochemistry was 0.898. High level MSI (MSI-H) status was correlated with the Tumor, Node, Metastasis stage, tumor location site, metastasis, tumor grade, mucinous histological type and BRAF-type mutations. Conclusion: The NCI panel PCR assay has excellent sensitivity and specificity for detecting MSI in an East Asian population.