A novel PCR-based genotyping scheme for clinical Klebsiella pneumoniae-Reference-Cited by-同舟云学术

A novel PCR-based genotyping scheme for clinical Klebsiella pneumoniae

Published:2014-01 Issue:1 Volume:9 Page:21-32
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Chen Zhenhong¹,Liu Mengying²³,Cui Yujun²,Wang Li²³,Zhang Yiquan²,Qiu Jingfu³,Yang Ruifu²⁴,Liu Changting⁵,Zhou Dongsheng⁶

Affiliation:

1. Nanlou Respiratory Diseases Department, Chinese People’s Liberation Army General Hospital, Beijing 100853, China

2. State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China

3. School of Public Health, Chongqing Medical University, Chongqing 400016, China

4. School of Medicine, Tsinghua University, Bejing 100084, China

5. Nanlou Respiratory Diseases Department, Chinese People’s Liberation Army General Hospital, Beijing 100853, China.

6. State Key Laboratory of Pathogen & Biosecurity, Beijing Institute of Microbiology & Epidemiology, Beijing 100071, China.

Abstract

ABSTRACT: Aim: To establish a PCR-based genotyping method for clinical Klebsiella pneumoniae. Materials & methods: The prevalence of six serotype markers, 41 large variably presented gene clusters, and seven additional virulence markers were screened by PCR in 327 clinical K. pneumoniae strains from China. Results: Detection of serotype markers enabled the identification of capsular serotypes K1, K2, K5, K20, K54 and K57. K. pneumoniae isolates of different origins gave distinct profiles of virulence loci, allowing us to gain a full overview of virulence gene distribution of the strains tested. A novel genotyping scheme was established to group clinical K. pneumoniae strains into distinct complexes based on the profiles of large variably presented gene clusters and virulence markers. Conclusion: This PCR-based genotyping method would be useful to not only characterize genetic diversity and virulence gene distribution, but also for genotyping, origin tracing and risk estimation of K. pneumoniae.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb.13.137

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