Rapid and reliable screening of HLA-B*15:02 in four Chinese populations using single-tube multiplex real-time PCR assay-Reference-Cited by-同舟云学术

Rapid and reliable screening of HLA-B*15:02 in four Chinese populations using single-tube multiplex real-time PCR assay

Published:2017-06 Issue:9 Volume:18 Page:853-963
ISSN:1462-2416
Container-title:Pharmacogenomics
language:en
Short-container-title:Pharmacogenomics

Author:

Wang Huijuan¹²,Kang Xing¹,Zhou Shaohe¹,Chen Rong¹,Liu Zhengbin¹,Han Min¹,Chen Chao¹,Gong Yongkuan²

Affiliation:

1. The National Engineering Research Center for Miniaturized Detection Systems, College of Life Science, Northwest University, Xi’an, China

2. Key Laboratory of Synthetic & Natural Function Molecular Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi’an, China

Abstract

Aim: HLA-B*15:02 screening should be performed to prevent antiepileptic drug induced severe cutaneous adverse reactions in populations of Asian origin. This study aimed to develop fast and reliable HLA-B*15:02 genotyping method and to investigate the distribution of HLA-B*15:02 in different Chinese ethnicities. Materials & methods: A single-tube multiplex real-time PCR assay for HLA-B*15:02 genotyping was established by combining allele-specific primers with TaqMan probes. Results: The HLA-B*15:02 genotyping results in Bouyei (n = 100) by the established assay were completely consistent with the corresponding PCR sequence-based typing findings. The percentage of HLA-B*15:02 carrier in Bouyei (19%; n = 100) was significantly higher than those of Han (1%; n = 100), Tibetan (0%; n = 100) and Uyghur (0%; n = 50) populations (p < 0.001). Conclusion: The novel method provides rapid, reliable and cost-effective detection of HLA-B*15:02 allele in clinical applications.

Publisher

Future Medicine Ltd

Subject

Pharmacology,Genetics,Molecular Medicine

Link

https://www.futuremedicine.com/doi/pdf/10.2217/pgs-2017-0041

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