In silico identification of conserved cis-acting RNA elements in the SARS-CoV-2 genome-Reference-Cited by-同舟云学术

In silico identification of conserved cis-acting RNA elements in the SARS-CoV-2 genome

Published:2020-07 Issue:7 Volume:15 Page:409-417
ISSN:1746-0794
Container-title:Future Virology
language:en
Short-container-title:Future Virology

Author:

Alhatlani Bader Y¹^ORCID

Affiliation:

1. Department of Medical Applied Sciences, Unayzah Community College, Qassim University, Unayzah, Saudi Arabia

Abstract

Aim: The aim of this study was to computationally predict conserved RNA sequences and structures known as cis-acting RNA elements (CREs) in the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genome. Materials & methods: Bioinformatics tools were used to analyze and predict CREs by obtaining viral sequences from available databases. Results: Computational analysis revealed the presence of RNA stem-loop structures within the 3′ end of the ORF1ab region analogous to previously identified SARS-CoV genomic packaging signals. Alignment-based RNA secondary structure predictions of the 5′ end of the SARS-CoV-2 genome also identified conserved CREs. Conclusion: These CREs may be potential vaccine and/or antiviral therapeutic targets; however, further studies are warranted to confirm their roles in the SARS-CoV-2 life cycle.

Publisher

Future Medicine Ltd

Subject

Virology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fvl-2020-0163

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