Evidence of bacterial DNA presence in chorionic villi and amniotic fluid in the first and second trimester of pregnancy-Reference-Cited by-同舟云学术

Evidence of bacterial DNA presence in chorionic villi and amniotic fluid in the first and second trimester of pregnancy

Published:2021-07 Issue:11 Volume:16 Page:801-810
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Campisciano Giuseppina¹^ORCID,Quadrifoglio Mariachiara²^ORCID,Comar Manola¹³^ORCID,Seta Francesco De³⁴^ORCID,Zanotta Nunzia¹^ORCID,Ottaviani Chiara³,Barbieri Moira²^ORCID,Chiodo Antonella⁵,Stampalija Tamara²³^ORCID

Affiliation:

1. Unit of Advanced Microbiology Diagnosis & Translational Research, Institute for Maternal & Child Health, IRCCS Burlo Garofolo, Via dell'Istria 65/1, Trieste, 34137, Italy

2. Unit of Fetal Medicine & Prenatal Diagnosis, Institute for Maternal & Child Health, IRCCS Burlo Garofolo, Via dell'Istria 65/1, Trieste, 34137, Italy

3. Department of Medical, Surgical & Health Sciences, University of Trieste, Strada di Fiume 447, Trieste, 34149, Italy

4. Obstetrics & Gynecology, Institute for Maternal & Child Health, IRCCS Burlo Garofolo, Via dell'Istria 65/1, Trieste, 34137, Italy

5. Department of Obstetrics & Gynecology, Policlinico Universitario Duilio Casula, University of Cagliari, SS 554, 09042 Monserrato, Cagliari, Italy

Abstract

The sterile-womb dogma in uncomplicated pregnancy has been lively debated. Data regarding the in utero microbiome environment are based mainly on studies performed at the time of delivery. Aim: To determine whether human placenta and amniotic fluid are populated by a bacterial microbiota in the first and second trimesters of pregnancy. Materials & methods: We analyzed by next-generation sequencing method 24 and 29 samples from chorionic villus sampling (CVS) and amniocentesis (AC), respectively. The V3 region of the 16S rRNA gene was sequenced. Results: 37.5% of CVS and 14% of AC samples showed the presence of bacterial DNA. Conclusion: Our study suggests that bacterial DNA can be identified in the placenta and amniotic fluid during early prenatal life.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2020-0243

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