Synergistic activity of dobutamine combined with azoles and its mechanism of action against strains of<i>Candida glabrata</i>-Reference-Cited by-同舟云学术

Synergistic activity of dobutamine combined with azoles and its mechanism of action against strains ofCandida glabrata

Published:2022-04 Issue:6 Volume:17 Page:437-448
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Barroso Fatima DD¹,da Silva Lisandra J¹,Sá Lívia GAV¹²^ORCID,da Silva Cecília R¹^ORCID,Neto João BA¹²,do Nascimento Francisca BA¹,Queiroz Helaine A¹,Leitão Amanda C¹,Cabral Vitória PF¹,Rodrigues Daniel S¹,Barbosa Amanda D¹,Cavalcanti Bruno C³,Morais Manoel O³,Júnior Hélio VN¹^ORCID

Affiliation:

1. Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Ceará, Fortaleza, Brazil

2. Faculty of Biomedicine, Unichristus University Center, Fortaleza, Brazil

3. Drug Research and Development Center, Federal University of Ceará, Fortaleza, CE, 60430-276, Brazil

Abstract

Aims: To evaluate the antifungal effect of dobutamine against Candida glabrata as well as its synergism with azoles and its action on biofilm. Methods: The M27-A3 protocol and flow cytometry were used for elucidation of the possible mechanism of action. Results: The tested isolates presented MICs ranging from 2 to 32 μg/ml for dobutamine, with fungistatic effect. A total of 82% of the strains showed synergism with fluconazole, with 90% showing synergism with itraconazole. The effect on biofilm formation was nonsignificant. Cytometry tests showed that dobutamine induced mitochondrial depolarization. Conclusion: Dobutamine has an antifungal effect on strains of C. glabrata and synergistic activity with azoles. This effect is probably mediated by increased oxidative damage to the membrane.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2020-0256

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