Affiliation:
1. Unit of Microbiology, Department of Biomedical Sciences & Public Health, Polytechnic University of Marche Medical School, Ancona, Italy
2. Unit of Microbiology, Department of Life & Environmental Sciences, Polytechnic University of Marche, Ancona, Italy
Abstract
Aim: This study reports the characterization of carbapenem-resistant colonizing strains of K. pneumoniae. Methods: 650 stool samples were screened for carbapenem-resistant K. pneumoniae (CR-Kp). All strains were characterized for antibiotic susceptibility, typing features, main carbapenemases and extended-spectrum ß-lactamases. The carbapenemase transferability was assessed by interspecific conjugation. Results: Eighteen CR-Kp were multidrug resistant, five were KPC producing. A predominance of ST307 isolates, constituting the predominant cluster by PFGE analysis, was identified (50% were KPC-2 producers). Conjugation data showed the co-transfer of blaKPC-2, blaTEM-1, blaOXA-1, blaCTX-M-15 in a single large pKPN3-like plasmid. Conclusion: Our data pointed out the diversity of colonizing K. pneumoniae strains compared with clinical ones. The predominance of ST307 strains suggested an increased spreading, even in our area, of this high-risk clone.
Funder
Polytechnic University of Marche.
Subject
Microbiology (medical),Microbiology
Cited by
10 articles.
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