PanB over-representation as part of pyrazinamide action: a proteomic insight-Reference-Cited by-同舟云学术

PanB over-representation as part of pyrazinamide action: a proteomic insight

Published:2021-11 Issue:17 Volume:16 Page:1303-1308
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Barros Isabella Letícia Esteves¹^ORCID,Meneguello Jean Eduardo²^ORCID,Ghiraldi-Lopes Luciana Dias³,Arita Gláucia Sayuri²^ORCID,de Oliveira Silva João Vitor¹^ORCID,Ferracioli Katiany Rizzieri Caleffi²³^ORCID,de Lima Scodro Regiane Bertin¹³^ORCID,Siqueira Vera Lucia Dias²³^ORCID,Pilau Eduardo Jorge⁴^ORCID,Campanerut-Sá Paula Aline Zanetti¹³,Cardoso Rosilene Fressatti¹²³^ORCID

Affiliation:

1. Postgraduate Program in Health Sciences, State University of Maringá, 87020-900, PR, Brazil

2. Postgraduate Program in Biosciences & Physiopathology, State University of Maringá, 87020-900, PR, Brazil

3. Department of Clinical Analysis & Biomedicine, State University of Maringá, 87020-900, PR, Brazil

4. Department of Chemistry, State University of Maringá, 87020-900, PR, Brazil

Abstract

Background: Pyrazinamide (PZA) represents a milestone as a first-line antituberculosis drug due to its sterilizing activity against Mycobacterium tuberculosis. Materials & Methods: The protein changes induced by subinhibitory PZA exposure of M. tuberculosis in acidic pH were evaluated by a proteomic approach. Results: Among the 1059 M. tuberculosis proteins identified, the specific acidification in the culture medium induced the over-representation of MurF (Rv2157c), and its under-representation was induced by 12 h of PZA exposure. PanB (Rv2225) was over-represented at 24 h of PZA exposure. Conclusion: The authors highlight the over-representation of PanB in M. tuberculosis correlates of PZA action in acidic pH, reinforcing the role of the pantothenate pathway as a bacillus drug target to be explored.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2020-0307

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