Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease-Reference-Cited by-同舟云学术

Comparison of DNA extraction methods and real-time PCR assays for the molecular diagnostics of chronic Chagas disease

Published:2020-08 Issue:12 Volume:15 Page:1139-1145
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Silgado Aroa¹,Moure Zaira¹,de Oliveira Maykon T²,Serre-Delcor Núria³,Salvador Fernando⁴,Oliveira Inés³,Molina Israel⁴,Pumarola Tomàs¹,Ramírez Juan C⁵^ORCID,Sulleiro Elena¹

Affiliation:

1. Department of Microbiology, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, PROSICS Barcelona, Spain

2. Departamento de Clínica Médica, Unidade de Cardiologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo (FMRP-USP), Brazil

3. Tropical Medicine Unit Vall d´Hebron-Drassanes, Universitat Autònoma de Barcelona, PROSICS Barcelona, Spain

4. Department of Infectious Diseases, Vall d'Hebron University Hospital, Universitat Autònoma de Barcelona, PROSICS Barcelona, Spain

5. Instituto Nacional de Parasitología “Dr. Mario Fatala Chaben” (INP-ANLIS), Buenos Aires, Argentina

Abstract

Aim: This work aimed to compare the sensitivity of four protocols for the detection of Trypanosoma cruzi DNA in 98 blood samples from chronic Chagas disease patients. Materials & methods: Two DNA extraction (automated and manual) methods and two T. cruzi satellite DNA qPCRs (with a recent design and the usually used set of primers) were analyzed. Results: Both DNA extraction methods and qPCR assays tested in this work gave comparable qualitative results, although the lowest Ct values were obtained when samples were analyzed using the new set of primers for T. cruzi satellite DNA. Conclusion: Our results encourage the implementation of automated DNA extraction systems and the new T. cruzi qPCR for the molecular diagnostics and treatment response monitoring of chronic Chagas disease patients.

Funder

Seventh Framework Programme

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2020-0023

Reference32 articles.

1. WHO. Chagas disease (American trypanosomiasis) (2020). http://www.who.int/news-room/fact-sheets/detail/chagas-disease-(american-trypanosomiasis)

2. Epidemiology of Chagas disease in non endemic countries: the role of international migration

3. Chagas disease

4. Randomized Trial of Posaconazole and Benznidazole for Chronic Chagas' Disease

5. Biological markers for evaluating therapeutic efficacy in Chagas disease, a systematic review

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Serial ‘deep-sampling’ PCR of fragmented DNA reveals the wide range ofTrypanosoma cruziburden among chronically infected hosts and allows accurate monitoring of parasite load following treatment;2024-06-10

2. Performance Evaluation of a Commercial Real-Time PCR Assay and of an In-House Real-Time PCR for Trypanosoma cruzi DNA Detection in a Tropical Medicine Reference Center, Northern Italy;Microorganisms;2020-10-30