Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona-Reference-Cited by-同舟云学术

Multilocus sequence typing of Treponema pallidum subsp. pallidum in Barcelona

Published:2021-09 Issue:13 Volume:16 Page:967-976
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Fernández-Naval Candela¹^ORCID,Arando Maider²^ORCID,Espasa Mateu³^ORCID,Antón Andrés¹^ORCID,Fernández-Huerta Miguel⁴^ORCID,Silgado Aroa¹^ORCID,Pinatar Cristina¹,Zarzuela Francesc²^ORCID,González-López Juan José¹^ORCID,Serra-Pladevall Judit¹^ORCID,Sulleiro Elena¹^ORCID,Pumarola Tomàs¹^ORCID,Vall-Mayans Martí⁵^ORCID,Esperalba Juliana¹^ORCID

Affiliation:

1. Microbiology Department, Hospital Univeristari Vall d'Hebron – Vall d'Hebron Research Institute, Universitat Autònoma de Barcelona, Passeig de la Vall d'Hebron, 119-129, 08035, Barcelona, Spain

2. Sexually Transmitted Infections Unit Vall d'Hebron-Drassanes, Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Carrer de Sant Oleguer, 17, 08001, Barcelona, Spain

3. Microbiology Department, Corporació Sanitària Parc Taulí, Parc del Taulí, 1, 08208, Sabadell, Barcelona, Spain

4. Microbiology Department, Hospital Universitari de Bellvitge, L'Hospitalet de Llobregat, Carrer de la Feixa Llarga, L'Hospitalet de Llobregat, 08907, Barcelona, Spain

5. Microbiology Department, Hospital Germans Trias i Pujol, Carretera de Canyet, 08916, Badalona, Barcelona, Spain

Abstract

Aim: To implement the multilocus sequence typing (MLST) methodology in syphilis samples previously characterized by enhanced CDC typing (ECDCT) and macrolide resistance. Materials & methods: MLST was performed on genital ulcer and blood samples by analyzing a region of the tp0136, tp0548 and tp0705 loci using Sanger sequencing. Results: Up to 59/85 (69.4%) of genital ulcer and 4/39 (10.3%) of whole blood samples were fully typed. The most frequent profiles were 1.3.1 (56%) and 1.1.1 (11%). All the 1.3.1 samples typed carried the A2058G mutation, responsible for macrolide resistance. MLST and ECDCT showed similar overall typing yields. Conclusion: Several allelic profiles of T. pallidum subsp. pallidum were identified and classified into two major genetic clades in Barcelona. Our results were similar to that described in Europe.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2021-0037

Reference34 articles.

1. Molecular Subtyping of Treponema pallidum Subspecies pallidum

2. Enhanced Molecular Typing ofTreponema pallidum: Geographical Distribution of Strain Types and Association with Neurosyphilis

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