Rescue of susceptibility to second-line drugs in resistant clinical isolates of <i>Mycobacterium tuberculosis</i>-Reference-Cited by-同舟云学术

Rescue of susceptibility to second-line drugs in resistant clinical isolates of Mycobacterium tuberculosis

Published:2022-05 Issue:7 Volume:17 Page:511-527
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Maringolo-Ribeiro Camila¹^ORCID,Grecco Júlia A¹,Bellato Débora L¹,Almeida Aryadne L²,Baldin Vanessa P²,Caleffi-Ferracioli Katiany R²^ORCID,Pavan Fernando R¹^ORCID

Affiliation:

1. São Paulo State University (UNESP), School of Pharmaceutical Sciences, Tuberculosis Research Laboratory, Araraquara, São Paulo, Brazil

2. State University of Maringá (UEM), Department of Clinical Analysis & Biomedicine, Maringá, Paraná, Brazil

Abstract

Aim: Antibiotic resistance is one of the biggest threats to global health, and this study aimed better understand how the efflux pumps are related to this process in tuberculosis clinical isolates. Results: The combination of antibiotics plus efflux pumps (EP) inhibitors was able to restore the susceptibility of clinical isolates in 100% of aminoglycosides resistance and 33.3% of the fluoroquinolones resistance. The relative expression of EP genes in pre-extensively drug-resistant isolates showed an increase of up to 1000-times. Conclusion: The rescue of susceptibility in the presence of EP inhibitors, the increased of activity and expression of the EP genes alert that the inhibition of EP can reduce the selection of resistant strains and improve treatment.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de São Paulo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2021-0215

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