Characterization and<i>in vitro</i>testing of newly isolated lytic bacteriophages for the biocontrol of<i>Pseudomonas aeruginosa</i>-Reference-Cited by-同舟云学术

Characterization andin vitrotesting of newly isolated lytic bacteriophages for the biocontrol ofPseudomonas aeruginosa

Published:2022-01 Issue:2 Volume:17 Page:111-141
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Harada Liliam K¹^ORCID,Silva Erica C¹^ORCID,Rossi Fernando PN²^ORCID,Cieza Basilio³,Oliveira Thais J¹^ORCID,Pereira Carla⁴^ORCID,Tomazetto Geizecler⁵^ORCID,Silva Bianca B¹^ORCID,Squina Fabio M¹^ORCID,Vila Marta MDC¹^ORCID,Setubal João C²^ORCID,Ha Taekjip³^ORCID,da Silva Aline M²^ORCID,Balcão Victor M¹⁴^ORCID

Affiliation:

1. PhageLab – Laboratory of Biofilms & Bacteriophages, University of Sorocaba, Sorocaba/SP, Brazil

2. Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, Brazil

3. Department of Biophysics & Biophysical Chemistry, Johns Hopkins University, Baltimore, MD, USA

4. Department of Biology & CESAM, University of Aveiro, Campus Universitário de Santiago, Aveiro, Portugal

5. Department of Engineering, Biological & Chemical Engineering Section (BCE), Aarhus University, Aarhus, Denmark

Abstract

Aim: Two lytic phages were isolated using P. aeruginosa DSM19880 as host and fully characterized. Materials & methods: Phages were characterized physicochemically, biologically and genomically. Results & conclusion: Host range analysis revealed that the phages also infect some multidrug-resistant (MDR) P. aeruginosa clinical isolates. Increasing MOI from 1 to 1000 significantly increased phage efficiency and retarded bacteria regrowth, but phage ph0034 (reduction of 7.5 log CFU/ml) was more effective than phage ph0031 (reduction of 5.1 log CFU/ml) after 24 h. Both phages belong to Myoviridae family. Genome sequencing of phages ph0031 and ph0034 showed that they do not carry toxin, virulence, antibiotic resistance and integrase genes. The results obtained are highly relevant in the actual context of bacterial resistance to antibiotics.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2021-0027

Reference116 articles.

1. Antimicrobial resistance: a global multifaceted phenomenon

2. Antimicrobial resistance: A global emerging threat to public health systems