Application of artificial neural networks to link genetic and environmental factors to DNA methylation in colorectal cancer

Author:

Coppedè Fabio123,Grossi Enzo45,Lopomo Angela16,Spisni Roberto7,Buscema Massimo58,Migliore Lucia123

Affiliation:

1. Department of Translational Research & New Technologies in Medicine & Surgery, Division of Medical Genetics, University of Pisa, Medical School, Via Roma 55, 56126 Pisa, Italy

2. Istituto Toscano Tumori (ITT), Florence, Italy

3. Interdepartmental Research Center Nutrafood ‘Nutraceuticals & Food for Health’, Pisa, Italy

4. Bracco Foundation, Milan, Italy

5. Semeion Research Center, Rome, Italy

6. Doctoral School in Genetics, Oncology & Clinical Medicine, University of Siena, Siena, Italy

7. Department of Surgery, Medical, Molecular & Critical Area Pathology, University of Pisa, Pisa, Italy

8. Department of Mathematical & Statistical Sciences, University of Colorado at Denver, CO, USA

Abstract

Aims: We applied artificial neural networks (ANNs) to understand the connections among polymorphisms of genes involved in folate metabolism, clinico-pathological features and promoter methylation levels of MLH1, APC, CDKN2AINK4A, MGMT and RASSF1A in 83 sporadic colorectal cancer (CRC) tissues, and to link dietary and lifestyle factors with gene promoter methylation. Materials & Methods: Promoter methylation was assessed by means of methylation-sensitive high-resolution melting and genotyping by PCR-RFLP technique. Data were analyzed with the Auto Contractive Map, a special kind of ANN able to define the strength of the association of each variable with all the others and to visually show the map of the main connections. Results: We observed a strong connection between the low methylation levels of the five CRC genes and the MTR 2756AA genotype. Several other connections were revealed, including those between dietary and lifestyle factors and the methylation levels of CRC genes. Conclusion: ANNs revealed the complexity of the interconnections among factors linked to DNA methylation in CRC.

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

Cited by 25 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3