Novel DNA CHIP detects human papillomaviruses in tonsillar tumors

Abstract

Aim: In this study, the novel VisionArray HPV assay was compared with the LCD CHIP array for the detection of HPVs in tonsillar carcinoma. Materials & methods: A Panel of tonsillar tumors and control samples containing know HPV isolates were tested with the VisonArry HPV Assay (Zytovision, Bremerhaven, Germany) and the Chipron MycoDirect LCD Assay (Chipron, Berlin, Germany). Results: While both assays enabled the discrimination between low-risk, intermediate and high-risk types, the LCD CHIP assay had a better laboratory performance and was more comfortable in its usage. However, both arrays delivered comparable results. Conclusion: Both assays are appropriate tools for the detection of clinically relevant HPV strains in tonsillar tumors. However, if the LCD CHIP array is established, the more complex workflow required for the VisionArray HPV assay could restrain diagnostic labs from a change to this novel assay despite its good quality.