Procedures for the expansion of CD14+ precursors from acute myeloid leukemic cells to facilitate dendritic cell-based immunotherapy

Author:

van den Ancker Willemijn1,Wijnands Pepijn GJB2,Ruben Jurjen M3,Westers Theresia M3,Punt Bianca3,Bachas Costa3,Ravenshorst Niek2,van Wetering Sandra2,Kruisbeek Ada M2,Bontkes Hetty J3,Ossenkoppele Gert J3,van de Loosdrecht Arjan A3,de Gruijl Tanja D4

Affiliation:

1. Department of Hematology, VU University Medical Center, VU Institute for Cancer & Immunology, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands.

2. DCPrime BV, Leiden, The Netherlands

3. Department of Hematology, VU University Medical Center, VU Institute for Cancer & Immunology, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

4. Department of Medical Oncology, VU Institute for Cancer & Immunology, VU University Medical Center, Amsterdam, The Netherlands

Abstract

Aims: Vaccination with acute myeloid leukemia (AML)-derived dendritic cells (DCs) is a promising immunotherapeutic approach to prevent relapse of AML. However, in clinical practice AML-derived DC culture is unfeasible in 40% of cases. Here, we demonstrate that AML cells can be expanded in vitro prior to differentiation with cocktails of cytokines with known myeloid growth-promoting effects. Results: Nine out of 13 initially CD14- samples gain de novo CD14 (>10%) expression (69% increment; p = 0.01) after in vitro expansion. These expanded CD14+ leukemic cells displayed a high probability (six out of six initially CD14- samples tested) to differentiate into DCs upon culture with GM-CSF, TNF-α and IL-4. Conclusion: Induction of CD14 on initially CD14- AML cells potentially increases the number of patients eligible for DC-based immunotherapy.

Publisher

Future Medicine Ltd

Subject

Oncology,Immunology,Immunology and Allergy

Reference26 articles.

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