The epitope sequence of S16, a monoclonal antibody against influenza C virus hemagglutinin-esterase fusion glycoprotein-Reference-Cited by-同舟云学术

The epitope sequence of S16, a monoclonal antibody against influenza C virus hemagglutinin-esterase fusion glycoprotein

Published:2017-03 Issue:3 Volume:12 Page:93-101
ISSN:1746-0794
Container-title:Future Virology
language:en
Short-container-title:Future Virology

Author:

Okuwa Takako¹,Sasaki Yutaka²,Matsuzaki Yoko³,Himeda Toshiki¹,Yoshino Naoto²,Hongo Seiji³,Ohara Yoshiro¹⁴,Muraki Yasushi¹²

Affiliation:

1. Department of Microbiology, Kanazawa Medical University School of Medicine, Uchinada, Ishikawa, Japan

2. Division of Infectious Diseases & Immunology, Department of Microbiology, School of Medicine, Iwate Medical University, Yahaba, Iwate, Japan

3. Department of Infectious Diseases, Yamagata University Faculty of Medicine, Iida-Nishi, Yamagata, Japan

4. Present address: Yamagata Kosei Hospital, 255 Onigoe, Sugesawa, Yamagata 990–2362, Japan

Abstract

Aim: S16, a monoclonal antibody against the hemagglutinin-esterase fusion (HEF) glycoprotein of influenza C virus, reacts with SV40 large T antigen (LT) and a host cellular component(s). The aim is to determine the location of S16 linear epitope on LT and the amino acid sequence of S16 epitope. Materials & methods: BHK-21 cells expressing wild-type and mutant LTs, HEFs or GFPs, each of which was tagged with a FLAG epitope, were analyzed by immunoblotting using S16. Results & conclusions: An amino acid sequence 98-FNEENL-103 on LT forms a linear epitope recognized by S16. The sequence of S16 epitope was defined as F[NAT]EE[NYA]L, excluding FAEEAL and FTEEAL. This finding will be of help in identifying a host cellular component(s) crossreactive with S16.

Publisher

Future Medicine Ltd

Subject

Virology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fvl-2016-0105

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