Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme-Reference-Cited by-同舟云学术

Biomimetic keratin gold nanoparticle-mediated in vitro photothermal therapy on glioblastoma multiforme

Published:2021-01 Issue:2 Volume:16 Page:121-138
ISSN:1743-5889
Container-title:Nanomedicine
language:en
Short-container-title:Nanomedicine

Author:

Guglielmelli Alexa¹²^ORCID,Rosa Paolo³^ORCID,Contardi Marco⁴^ORCID,Prato Mirko⁵^ORCID,Mangino Giorgio³^ORCID,Miglietta Selenia⁶^ORCID,Petrozza Vincenzo³^ORCID,Pani Roberto³^ORCID,Calogero Antonella³^ORCID,Athanassiou Athanassia⁴^ORCID,Perotto Giovanni⁴^ORCID,De Sio Luciano²³^ORCID

Affiliation:

1. Department of Physics, University of Calabria, Arcavacata di Rende, Cosenza 87036, Italy

2. CNR-Lab. LiCryl, Institute NANOTEC, Arcavacata di Rende 87036, Italy

3. Department of Medico-surgical Sciences & Biotechnologies, Center for Biophotonics, Sapienza University of Rome, Corso della Repubblica, Latina 79, 04100, Italy

4. Smart Materials, Istituto Italiano di Tecnologia, Via Morego, Genoa 30, 16163, Italy

5. Materials Characterization Facility, Istituto Italiano di Tecnologia, Via Morego, Genova 30, 16163, Italy

6. Department of Anatomy, Histology, Forensic Medicine & Orthopaedics, Sapienza University of Rome, Via Alfonso Borelli, Rome 50, 00161, Italy

Abstract

Aim: To realize and characterize a new generation of keratin-coated gold nanoparticles (Ker-AuNPs) as highly efficient photosensitive nanosized therapeutics for plasmonic photothermal (PPT) therapy. Materials & methods: The chemical, physical, morphological and photothermal properties of Ker-AuNPs are investigated using dynamic light scattering, ζ-potential, UV–Visible, Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, transmission electron microscopy and high-resolution thermography. In vitro experiments are performed on a human glioblastoma cell line (i.e., U87-MG), using viability assays, transmission electron microscopy, fluorescence microscopy, cytometric analyses and PPT experiments. Results: Experiments confirm the excellent biocompatibility of Ker-AuNPs, their efficient cellular uptake and localized photothermal heating capabilities. Conclusion: The reported structural and functional properties pointed out these Ker-AuNPs as a promising new tool in the field of biocompatible photothermal agents for PPT treatments against cancer-related diseases.

Funder

Air Force Office of Scientific Research

Fondazione Cariplo

Sapienza Università di Roma

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/nnm-2020-0349

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1. Photothermal Efficiencies of Nanoshells and Nanorods for Clinical Therapeutic Applications