Apoptosis pathway in the combined treatment of x-ray and 5-FU-loaded triblock copolymer-coated magnetic nanoparticles

Author:

Golbaz Rezvan12,Khoei Samideh32ORCID,Khoee Sepideh4,Shirvalilou Sakine3,Safa Majid5,Mahdavi Seied R2,Karimi Mohammad R4

Affiliation:

1. Student Research Committee, Iran University of Medical Sciences, Tehran, Iran

2. Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

3. Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran

4. Department of Polymer Chemistry, School of Chemistry, College of Science, University of Tehran, Tehran, Iran

5. Department of Hematology & Blood Transfusion, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran

Abstract

Aim: In this study, the effects of ionizing radiation and 5-fluorouracil (5-FU)-loaded triblock copolymer-coated magnetic nanoparticles (NPs) on the induction of apoptosis in HT-29 and HCT-116 were investigated. Materials & methods: The percentage of apoptotic cells and alteration of the expression of apoptotic-related proteins were evaluated in treated cells by flow cytometry and western blot analysis, respectively. Results: Combination treatment with 5-FU and radiation had a stronger effect on decreasing Bcl-2 expression and increasing expression of Bax, cleaved caspase-9, cleaved caspase-3, cleaved PARP compared with each treatment alone. Conclusion: The combination of radiation and triblock copolymer-coated magnetic NPs as 5-FU drug carriers works by triggering apoptosis to improve in vitro treatment efficacy. Additional study may present the NPs as an effective approach for the treatment of colon cancer.

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

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