Differential dose-dependent effects of zinc oxide nanoparticles on oxidative stress-mediated pancreatic β-cell death

Abstract

Aim: To study the effects of zinc oxide nanoparticles (ZON) on oxidative stress-mediated pancreatic β-cell death. Methods: Cellular uptake of ZON, effects on antioxidant factors and apoptosis were studied. Results: ZON get internalized by endocytosis and increase intracellular zinc ion levels. ZON treatment (30 and 100 μg/ml) to RIN5f cells resulted in cytotoxicity, oxidative stress and apoptosis. ZON (1, 3, 10 μg/ml, subcytotoxic concentrations) increased super oxide dismutase activity and levels of reduced glutathione in RIN5f cells. Furthermore, ZON (subcytotoxic concentrations) protected RIN5f cells from H2O2-induced oxidative stress as evidenced by reduced reactive oxygen species levels; increased super oxide dismutase activity and glutathione levels; and reduced apoptotic death. Conclusion: ZON (subcytotoxic concentrations) protect pancreatic β cells from oxidative-stress-mediated cell death.