Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance-Reference-Cited by-同舟云学术

Stem cell labeling with iron oxide nanoparticles: impact of 3D culture on cell labeling maintenance

Published:2016-08 Issue:15 Volume:11 Page:1957-1970
ISSN:1743-5889
Container-title:Nanomedicine
language:en
Short-container-title:Nanomedicine

Author:

Kilian Teresa¹,Fidler Florian²,Kasten Annika³,Nietzer Sarah⁴,Landgraf Veronika⁴,Weiß Katrin⁴,Walles Heike¹⁴,Westphal Fritz⁵,Hackenberg Stephan⁶,Grüttner Cordula⁵,Steinke Maria¹⁴

Affiliation:

1. Fraunhofer Institute for Interfacial Engineering & Biotechnology IGB, Translational Center “Regenerative Therapies for Oncology & Musculoskeletal Diseases” – Würzburg branch, Röntgenring 11, 97070 Würzburg, Germany

2. Research Center Magnetic-Resonance-Bavaria, Am Hubland, 97074 Würzburg, Germany

3. Department of Oral & Maxillofacial Surgery, Facial Plastic Surgery, Rostock University Medical Center, Schillingallee 35, 18057 Rostock, Germany

4. Tissue Engineering & Regenerative Medicine, University Hospital Würzburg, Röntgenring 11, 97070 Würzburg, Germany

5. Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Straße 4, 18119 Rostock, Germany

6. Department of Oto-Rhino-Laryngology, Plastic, Aesthetic & Reconstructive Head & Neck Surgery, University Hospital Würzburg, Josef-Schneider-Straße 11, 97080 Würzburg, Germany

Abstract

Aim: We aimed to analyze the suitability of nanoparticles (M4E) for safe human mesenchymal stem cell (hMSC) labeling and determined cell labeling maintenance in 2D and 3D culture. Materials & methods: We investigated cell–particle interaction and the particles’ impact on cell viability, growth and proliferation. We analyzed cell labeling maintenance in 2D and 3D culture invasively and noninvasively. Results: M4E do not affect cell viability, growth and proliferation and do not cause chromosomal aberrations. Cell labeling maintenance is up to five-times higher in 3D conditions compared with 2D culture. Conclusion: M4E allow safe hMSC labeling and noninvasive identification. Our hMSC-loaded, 3D tissue-engineered construct could serve as a graft for regenerative therapies, in which M4E-labeled hMSCs can migrate to their target.

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/nnm-2016-0042

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