Synthesis and characterization of vitamin D3-functionalized carbon dots for CRISPR/Cas9 delivery-Reference-Cited by-同舟云学术

Synthesis and characterization of vitamin D3-functionalized carbon dots for CRISPR/Cas9 delivery

Published:2021-08 Issue:19 Volume:16 Page:1673-1690
ISSN:1743-5889
Container-title:Nanomedicine
language:en
Short-container-title:Nanomedicine

Author:

Hasanzadeh Akbar¹²³,Radmanesh Fatemeh⁴⁵,Hosseini Elaheh Sadat¹²,Hashemzadeh Iman¹²³,Kiani Jafar⁶⁷,Naseri Marzieh⁶⁷,Nourizadeh Helena³,Fatahi Yousef⁸⁹¹⁰,Azar Behjat Kheiri Yeghaneh⁷¹¹,Marani Behnaz Golnari¹²³,Beyzavi Ali¹²,Mahabadi Vahid Pirhajati¹¹³,Karimi Mahdi¹²³⁶¹⁴^ORCID

Affiliation:

1. Cellular & Molecular Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran

2. Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran

3. Advanced Nanobiotechnology & Nanomedicine Research Group (ANNRG), Iran University of Medical Sciences, Tehran, 1449614535, Iran

4. Uro-Oncology Research Center, Tehran University of Medical Sciences, Tehran, 1449614535, Iran

5. Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology & Technology, ACECR, Tehran, 1665659911, Iran

6. Oncopathology Research Center, Iran University of Medical Sciences, Tehran, 1449614535, Iran

7. Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran

8. Nanotechnology Research Centre, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

9. Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

10. Universal Scientific Education & Research Network (USERN), Tehran, 1417755331, Iran

11. Student Research Committee, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, 1449614535, Iran

12. Department of Mechanical Engineering, Boston University, Boston, MA 02215, USA

13. Neuroscience research center, Iran University of medical sciences, Tehran, 1449614535, Iran

14. Research Center for Science & Technology in Medicine, Tehran University of Medical Sciences, Tehran, 1417613151, Iran

Abstract

Aim: To develop a novel nanovector for the delivery of genetic fragments and CRISPR/Cas9 systems in particular. Materials & methods: Vitamin D3-functionalized carbon dots (D/CDs) fabricated using one-step microwave-aided methods were characterized by different microscopic and spectroscopic techniques. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and flow cytometry were employed to determine the cell viability and transfection efficiency. Results: D/CDs transfected CRISPR plasmid in various cell lines with high efficiency while maintaining their remarkable efficacy at high serum concentration and low plasmid doses. They also showed great potential for the green fluorescent protein disruption by delivering two different types of CRISPR/Cas9 systems. Conclusion: Given their high efficiency and safety, D/CDs provide a versatile gene-delivery vector for clinical applications.

Funder

Iran University of Medical Sciences

Publisher

Future Medicine Ltd

Subject

Development,General Materials Science,Biomedical Engineering,Medicine (miscellaneous),Bioengineering

Link

https://www.futuremedicine.com/doi/pdf/10.2217/nnm-2021-0038

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2. Development and Applications of CRISPR-Cas9 for Genome Engineering

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