<i>Akkermansia muciniphila</i> plays a neuroprotective role in HMC3 cells through the ‘gut

Akkermansia muciniphila plays a neuroprotective role in HMC3 cells through the ‘gut–brain’ axis

Published:2023-03 Issue:5 Volume:18 Page:255-266
ISSN:1746-0913
Container-title:Future Microbiology
language:en
Short-container-title:Future Microbiology

Author:

Zou Rong¹²,Shen Guohong³,Wu Yixiao¹,Guo Min¹,Chen Jun⁴,Yang Sheng⁴,Zhao Hongyang³,Zheng Huajun¹

Affiliation:

1. NHC Key Laboratory of Reproduction Regulation (Shanghai Institute for Biomedical & Pharmaceutical Technologies), Fudan University, Shanghai, 200032, China

2. Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, 200240, China

3. Department of Pediatrics, Central Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250013, China

4. CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

Abstract

Aims: We investigated the neuroprotective effects of Akkermansia muciniphila through the ‘gut–brain’ axis. Methods: Human colon cancer (Caco-2) cells treated with A. muciniphila metabolites were used to create the conditioned medium from Caco-2 cells treated with A. muciniphila metabolites (AC medium) medium, then treated human microglial clone 3 (HMC3) cells to simulate the gut–brain axis in vitro. Bioinformatics analyses were performed to investigate the molecular mechanisms by which the AC medium affected HMC3 cells. Results: The secretion of inflammatory cytokines IL-6 (0.37 ± 0.80-fold) and IL-17A (0.05 ± 0.18-fold) by HMC3 cells was inhibited by the AC medium. Differentially expressed genes were mainly enriched in immune-related signaling pathways, such as the cAMP and TGF-β signaling pathways. Conclusion: A. muciniphila might be a source of therapeutic approaches to alleviate microglia-mediated neuroinflammatory diseases.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fmb-2022-0007

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