Cytokine profile in HTB-4 cells with respect to fimH, papC and hlyA expression in uropathogenic Escherichia coli invasion

Author:

Koley Snehashis1ORCID,Mukherjee Mandira1ORCID

Affiliation:

1. Department of Biochemistry & Medical Biotechnology, School of Tropical Medicine, Kolkata, 700073, India

Abstract

Background: In this study, uropathogenic Escherichia coli (UPEC) from pregnant and nonpregnant patients were characterized with respect to antimicrobial resistance (AMR) and expression of their virulence factors and cytokines elicited upon infection in urothelial (HTB-4) cells in vitro to frame proper therapeutics. Methods: Antibiotic sensitivity and adherence to HTB-4 cells were tested and PCR and real-time PCR were performed. Results: UPEC from nonpregnant patients showed the most resistance with a significant correlation between the expression of hlyA and  TGF-β and  papC and GCSF. The expression of fimH and  IFN-γ, fimH and  IL-1β, and fimH and  IL-17A, respectively, were significantly correlated in UPEC from pregnant patients. Conclusion: Cytokine expression profiles were correlated with the expression of virulence genes in UPEC that was isolated from different populations, and should be accounted for along with AMR analysis.

Publisher

Future Medicine Ltd

Subject

Microbiology (medical),Microbiology

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