Enhancing medial layer recellularization of tissue-engineered blood vessels using radial microchannels

Author:

Eufrásio-da-Silva Tatiane123,Ruiz-Hernandez Eduardo34,O’Dwyer Joanne156,Picazo-Frutos Dolores15,Duffy Garry P1236,Murphy Bruce P237

Affiliation:

1. Department of Anatomy, Tissue Engineering Research Group (TERG), Royal College of Surgeons in Ireland (RCSI), Dublin, Ireland

2. Trinity Centre for Biomedical Engineering (TCBE), Trinity Biomedical Sciences Institute, TCD, Dublin, Ireland

3. Advanced Materials & BioEngineering Research Centre (AMBER), RCSI & TCD, Dublin, Ireland

4. School of Pharmacy & Pharmaceutical Sciences, Trinity College Dublin (TCD), Dublin, Ireland

5. School of Pharmacy, RCSI, Dublin, Ireland

6. Anatomy, School of Medicine, College of Medicine Nursing & Health Sciences, National University of Ireland Galway, Galway, Ireland

7. Department of Mechanical & Manufacturing Engineering, TCD, Dublin, Ireland

Abstract

Aim: Cell repopulation of tissue-engineered vascular grafts (TEVGs) from decellularized arterial scaffolds is limited by dense concentric tunica media layers which impede cells migrating radially between the layers. We aimed to develop and validate a new microneedle device to modify decellularized carotid arteries with radial microchannels to enhance medial layer repopulation. Material & methods: Modified decellularized porcine arteries were seeded with rat mesenchymal stem cells using either standard longitudinal injection, or a dual vacuum-perfusion bioreactor. Mechanical tests were used to assess the arterial integrity following modification. Results & conclusion: The method herein achieved radial recellularization of arteries in vitro without significant loss of mechanical integrity, Thus, we report a novel method for successful radial repopulation of decellularized carotid artery-based tissue-engineered vascular grafts.

Publisher

Future Medicine Ltd

Subject

Embryology,Biomedical Engineering

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