Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran-Reference-Cited by-同舟云学术

Comparative and phylogenetic analysis of Epstein–Barr virus isolates in gastric cancer and chronic gastritis patients in Iran

Published:2023-11 Issue:16 Volume:18 Page:1075-1088
ISSN:1746-0794
Container-title:Future Virology
language:en
Short-container-title:Future Virology

Author:

Sarshari Behrang¹²^ORCID,Zareh-Khoshchehreh Raziyeh¹³^ORCID,Tavallaei Mehdi⁴,Rajabnia Mohsen⁵,Norooz Mohammad Tayefeh⁶,Mohebbi Seyed Reza⁷^ORCID,Ravanshad Mehrdad¹^ORCID

Affiliation:

1. Department of Medical Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2. Iranian Research Center for HIV/AIDS, Iranian Institute for Reduction of High-Risk Behaviors, Tehran University of Medical Sciences, Tehran, Iran

3. Food & Drug Administration, Iran Ministry of Health & Medical Education, Tehran, Iran

4. Colorectal Surgeon, Taleghani Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5. Non-Communicable Diseases Research Center, Alborz University of Medical Sciences, Karaj, Iran

6. Critical Care & Quality Improvement Research Center, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

7. Basic & Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology & Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Aim: To investigate the correlation between Epstein–Barr virus (EBV) variants and EBV-associated gastric cancer (EBVaGC) in Iran. Materials & methods: Biopsies from 21 gastric cancer (4 positive EBER-ISH [EBVaGC] and 17 negative EBER-ISH) and 11 gastritis patients were analyzed using nested PCR and sequencing. Results: Genotype 1 (75%) was the predominant EBV genotype. The P-thr variant was the most common in all groups (positive EBER-ISH 75%, negative EBER-ISH 76.4%, and gastritis 81.8%), followed by P-ala. Additionally, the predominant LMP1 strain in all groups was Med. Sequencing analysis revealed a strong correlation between the P-thr and type 1. Conclusion: Since no preferential correlations were observed between distinct EBV variants and the study groups, these results might indicate geographic polymorphisms.

Funder

Tarbiat Modares University

Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences

Publisher

Informa UK Limited

Subject

Virology

Link

https://www.futuremedicine.com/doi/pdf/10.2217/fvl-2023-0144

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