Epigenetic-mediated N-methyl-D-aspartate receptor changes in the brain of isolated reared rats-Reference-Cited by-同舟云学术

Epigenetic-mediated N-methyl-D-aspartate receptor changes in the brain of isolated reared rats

Published:2020-11 Issue:22 Volume:12 Page:1983-1997
ISSN:1750-1911
Container-title:Epigenomics
language:en
Short-container-title:Epigenomics

Author:

Loureiro Camila Marcelino¹²^ORCID,Fachim Helene Aparecida³⁴^ORCID,Corsi-Zuelli Fabiana⁴^ORCID,Shuhama Rosana⁴^ORCID,Joca Sâmia⁵⁶^ORCID,Menezes Paulo Rossi⁷^ORCID,Dalton Caroline F²^ORCID,Del-Ben Cristina Marta⁴^ORCID,Louzada-Junior Paulo¹^ORCID,Reynolds Gavin P²^ORCID

Affiliation:

1. Department of Internal Medicine, Division of Clinical Immunology. Ribeirão Preto Medical School, University of São Paulo, Brazil

2. Biomolecular Sciences Research Centre, Sheffield Hallam University, Sheffield, UK

3. Department of Endocrinology & Metabolism, Salford Royal Foundation Trust, Salford, UK

4. Department of Neurosciences & Behaviour, Ribeirão Preto Medical School, University of São Paulo, Brazil

5. Department of Biomolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Brazil

6. Aarhus Institute of Advanced Studies (AIAS), Aarhus University, Denmark

7. Department of Preventive Medicine, Faculty of Medicine, University of São Paulo, Brazil

Abstract

Aim: We investigated: Grin1, Grin2a, Grin2b DNA methylation; NR1 and NR2 mRNA/protein in the prefrontal cortex (PFC); and hippocampus of male Wistar rats exposed to isolation rearing. Materials & methods: Animals were kept isolated or grouped (n = 10/group) from weaning for 10 weeks. Tissues were dissected for RNA/DNA extraction and N-methyl-D-aspartate receptor subunits were analyzed using quantitative reverse transcription (RT)-PCR, ELISA and pyrosequencing. Results: Isolated-reared animals had: decreased mRNA in PFC for all markers, increased NR1 protein in hippocampus and hypermethylation of Grin1 in PFC and Grin2b in hippocampus, compared with grouped rats. Associations between mRNA/protein and DNA methylation were found for both brain areas. Conclusion: This study indicates that epigenetic DNA methylation may underlie N-methyl-D-aspartate receptor mRNA/protein expression alterations caused by isolation rearing.

Funder

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior

Fundação de Amparo à Pesquisa do Estado de São Paulo

Publisher

Future Medicine Ltd

Subject

Cancer Research,Genetics

Link

https://www.futuremedicine.com/doi/pdf/10.2217/epi-2020-0151

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